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New England Biolabs公司(NEB) - 企业动态 - 丁香通
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发布时间:2025-01-15
主页唯一 URLABRF 2014ABRF 2014
2014 年 3 月 22 日至 25 日,新墨西哥州阿尔伯克基 https://conf.abrf.org/ 欢迎光临我们的 317 号展台!
我们诚挚地邀请您参加我们的午餐研讨会,网址为今年的 ABRF 年会。周二 3 月 25 日 12:45 – 1:45pmRuidoso roomSave this event to your calendar
Next Generation Sequencing with NEB: Sample Preparation and PartneringDescription:Next Generation Sequencing (NGS) labs continue to be受到对更低输入量、更快和更高效方案的要求以及样品质量的挑战。与此同时,流程、试剂和 SOP 的简化变得越来越重要,测序服务的竞争也越来越激烈。考虑到核心实验室,本次研讨会将涵盖应对这些挑战的方法。
合作加强加州大学河滨分校的教学和研究格伦希克斯加州大学河滨分校NEBNext NGS 样品制备更新:ChIP-Seq、RNA-Seq、多重分析等。克里斯汀萨姆纳新英格兰Biolabs, Inc. 自动化 NGS 样品制备Fiona StewartNew England Biolabs, Inc. 请同时访问我们的海报使用定向 RNA 工作流程和组合复用方法实现 RNA 转录景观的高通量发现 Stewart, F. J. 等人。NGS 的快速高度复用解决方案使用低纳克 DNA InputSumner, C. 等人进行文库制备。New England Biolabs公司(NEB) New England Biolabs公司(NEB)已发布一种新DNA聚合酶:高保真的Q5(TM),它不仅提供了目前可用的保真度最高的克隆服务,也提供了覆盖最广扩增子(包括那些GC和AT富集区)的强大克隆功能。New England Biolabs公司开发出保真度超高和GC偏好最小的聚合酶 对于那些依据正确的DNA序列得出结果的实验,高保真克隆是必要的,如:SNP检测、蛋白表达和基因功能研究。然而, 如果DNA聚合酶不能均匀扩增不同类型的序列,那么克隆结果在正确性上将受到影响。例如,高保真PCR扩增GC富集区和其它挑战性的扩增子可导致产量下降,甚至非特异性扩增。此外,对于序列组装和\"缺失”序列而言,任何克隆偏好和下一代测序都能构成挑战,这一问题在测序领域内受到的关注日益增多。 NEB公司的新型聚合酶融合到加快进程的Sso7d DNA结合域,这能提高保真度(相比Taq酶提高了50倍)、速度和可靠性能(扩增对象的长度可达20 kb,而每kb的延伸时间却低到10秒)。Q5缓冲系统也获得大幅度优化,从而提高了覆盖GC和AT富集区以及其它挑战性扩增子的强大性能。 NEB产品营销经理Fiona Stewart博士称:\"高保真聚合酶往往更难于应对GC富集区和其它挑战性扩增子的PCR克隆。Q5聚合酶显著得降低了这些限制,并提出了高保真DNA扩增的性能。” 一种热启动版本的Q5的聚合酶利用合成性适体适用于室温,Q5和Q5热启动DNA聚合酶都可作为独立使用的酶,或者是方便性提高的2X master mix。不同的配剂已被专门开发以满足下一代测序库扩增的要求(NEBNext(®)高保真2X PCR Master Mix)。 多年来,发现和研究高保真聚合酶已成为NEB公司的一个研发重点。从1990年首次在Thermococcus litoralis中发现Vent® DNA聚合酶到最近商业化的Phusion ®DNA聚合酶,NEB公司的研究人员已识别和开发大量合成性能、速度和精度不同的高保值聚合酶。Q5 DNA聚合酶属于列单上最新和性能最高的酶。 关于New England Biolabs公司 New England Biolabs公司成立于20世纪70年代中期,在分子生物学酶行业内属于领导者,目前可提供最大的重组选择以及基因组研究的自主研发酶。NEB继续将产品范围扩大到PCR、基因表达、下一代测序中样本准备、细胞分析、表观遗传学和RNA分析。此外,NEB重视将代理合作,从而让新技术达到市场的关键领域。 【拜力核心代理品牌】 drummond eppendorf sigma-aldrich neb exocell abi agilent alfa atcc bioline brand cayman eMd ge公司 illumina invitrogen jackson leica millipore omega pall pierce santa cruz sbi sigma sigma公司 sigma 中国 thermo usp vector Corning 康宁 康宁公司 ENZO Meridian (拜力生物化学试剂网编辑整理:www.lookbio.com )本文链接: http://neb.immuno-online.com/view-1354641751.html
发布于 : 2025-01-15
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Protein Expression|Protein Purification|Protein Tools
DNA Modifying Enzymes and Cloning Technologies|Next Generation Sequencing Library Preparat
DNA修饰酶
DNA Assembly Cloning and Mutagenesis Kits
DNA修饰酶和克隆技术|NGS样品制备&;目标富集|PCR
缓冲液|下一代测序文库制备|NGS样品制备&;目标富集
Protein Expression|Protein Expression & Purification Technologies|Protein Purification
表观遗传学|限制性内切酶
DNA Modifying Enzymes and Cloning Technologies|Epigenetics
Nucleic Acid Purification|RNA Reagents
表观遗传学
DNA Modifying Enzymes and Cloning Technologies
Buffers|DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplificatio
Buffers|Markers & Ladders|RNA Reagents
Next Generation Sequencing Library Preparation|NGS Sample Prep & Target Enrichment
Nucleic Acid Purification|Protein Expression & Purification Technologies|RNA Reagents
Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno
Buffers|DNA Modifying Enzymes and Cloning Technologies
Buffers|Competent Cells
DNA Modifying Enzymes and Cloning Technologies|Next Generation Sequencing Library Preparat
Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno
Buffers
Buffers|Nucleic Acid Purification
Epigenetics|Protein Expression & Purification Technologies|Protein Purification
Epigenetics|Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplific
DNA质粒和底物
Markers & Ladders|RNA Reagents
感受态细胞|蛋白质表达|蛋白质表达&;净化技术|Prot
Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno
DNA组装、克隆和突变试剂盒
DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplification Techno
Competent Cells|Protein Expression|Protein Expression & Purification Technologies|Prot
Competent Cells|Protein Expression & Purification Technologies
蛋白质工具
Epigenetics|Next Generation Sequencing Library Preparation|NGS Sample Prep & Target En
Next Generation Sequencing Library Preparation|NGS Sample Prep & Target Enrichment|PCR
Glycobiology|Protein Tools
RNA试剂
Next Generation Sequencing Library Preparation|NGS Sample Prep & Target Enrichment|PCR
Protein Expression|Protein Expression & Purification Technologies|Protein Purification
基因表达
DNA Modifying Enzymes and Cloning Technologies|RNA Reagents
Cellular Analysis|DNA Plasmids & Substrates|Protein Tools
下一代测序的样品制备
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PCR,qPCR和扩增技术
DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplification Techno
限制性内切酶
主管细胞
Epigenetics|Next Generation Sequencing Library Preparation|NGS Sample Prep & Target En
DNA Modifying Enzymes and Cloning Technologies|Nucleic Acid Purification
PCR, Polymerases & Amplification Technologies
Protein Expression|Protein Expression & Purification Technologies
DNA Modifying Enzymes and Cloning Technologies|Next Generation Sequencing Library Preparat
Competent Cells|Protein Expression|Protein Expression & Purification Technologies|Prot
细胞分析
下一代测序文库制备|NGS样品制备&;目标富集|PCR
DNA质粒&;底物|蛋白质表达|蛋白质表达&;净化技术
核酸提纯
Epigenetics|NGS Sample Prep & Target Enrichment|PCR, Polymerases & Amplification T
DNA Modifying Enzymes and Cloning Technologies|Next Generation Sequencing Library Preparat
Buffers|Glycobiology
Nucleic Acid Purification|Protein Tools
NGS Sample Prep & Target Enrichment|PCR, Polymerases & Amplification Technologies|
Buffers|DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplificatio
Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno
Buffers|Competent Cells|Protein Expression|Protein Expression & Purification Technolog
Next Generation Sequencing Library Preparation|NGS Sample Prep & Target Enrichment|PCR
DNA Assembly Cloning and Mutagenesis Kits|DNA Modifying Enzymes and Cloning Technologies
Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno
Buffers|PCR, Polymerases & Amplification Technologies
DNA Modifying Enzymes and Cloning Technologies|Genome Editing
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Next Generation Sequencing Library Preparation
Cellular Analysis|Protein Tools
PCR, Polymerases & Amplification Technologies|Protein Expression|Protein Expression &a
Genome Editing|RNA Reagents
Cellular Analysis|Epigenetics|Protein Tools
DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplification Techno
Protein Expression & Purification Technologies|Protein Purification
Buffers|DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplificatio
Competent Cells|Protein Expression|Protein Expression & Purification Technologies
DNA Plasmids & Substrates|Protein Expression|Protein Expression & Purification Tec
Buffers|RNA Reagents
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DNA Plasmids & Substrates|Protein Expression|Protein Expression & Purification Tec
Nucleic Acid Purification|PCR, Polymerases & Amplification Technologies|RNA Reagents
DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplification Techno
Next Generation Sequencing Library Preparation|RNA Reagents
PCR, Polymerases & Amplification Technologies|RNA Reagents
Competent Cells|Protein Expression|Protein Expression & Purification Technologies|Prot
Protein Expression & Purification Technologies|RNA Reagents
基因组编辑
糖生物学
细胞分析|蛋白质表达&;纯化技术|蛋白质工具
Epigenetics|Next Generation Sequencing Library Preparation
DNA Plasmids & Substrates|Protein Expression|Protein Expression & Purification Tec
Buffers|Markers & Ladders|Protein Tools
DNA修饰酶和克隆技术|PCR,聚合酶&;放大技术
标记和梯子
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Buffers|Restriction Endonucleases
蛋白质表达与纯化技术
Buffers|Markers & Ladders
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