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neb内切酶_neb内切酶【价格,厂家,图片,批发,采购】
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发布时间:2025-01-15
HomeFAQs我应该如何为我的反应确定合适的退火温度?FAQ:我应该如何为我的反应确定合适的退火温度?请使用 NEB 的 Tm 计算器为您的引物对和感兴趣的聚合酶/缓冲液确定合适的退火温度。与其他计算器不同,NEB Tm 计算器在计算最佳退火温度时将影响熔化温度的缓冲成分考虑在内。其他在线计算器可能会低估最佳 Q5 退火温度。此资源的链接相关产品:OneTaq® Hot Start 2X Master Mix with Standard Buffer、Q5® High-Fidelity PCR Kit、OneTaq® Hot Start DNA Polymerase、OneTaq® Hot Start 2X Master Mix with GC Buffer、OneTaq®Quick-Load® 2X含标准缓冲液的预混液、Q5® 高保真 2X 预混液、Q5® 热启动高保真 2X 预混液、热启动 Taq DNA 聚合酶、热启动 Taq 2X 预混液、OneTaq® DNA Po聚酶,OneTaq® 2X Master Mix with Standard Buffer, OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer, OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer, Q5® High-Fidelity DNA Polymerase, Q5 ® 热启动高保真 DNA 聚合酶,M0500 Q5 Blood Direct 2X Master MixHomeFAQs使用Q5®高保真DNA聚合酶产品时,我的引物浓度应该是多少?常见问题:使用Q5®高保真DNA聚合酶产品时,我的引物浓度应该是多少?引物浓度一般应在200 nM和1 µM之间。建议使用 500 nM。此资源的链接相关产品:Q5® 高保真 PCR 试剂盒、Q5® 高保真 2X 预混液、Q5® 热启动高保真 2X 预混液、Q5® 高保真 DNA 聚合酶、Q5® Hot Start 高保真 DNA 聚合酶,M0500 Q5 Blood Direct 2X Master Mix主页帐户登录 NEB.com登录 NEB.com
丁香通为您找到 35 条 neb内切酶产品的详情参数,实时报价,价格行情,优质批发/供应等信息。您还可以找 neb内切酶,neb内切酶buff,sma1内切酶neb 等产品信息。
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主页常见问题解答 Faustovirus 加帽酶在 37°C 以外的温度下是否有活性?常见问题解答:Faustovirus 加帽酶在 37°C 以外的温度下是否有活性?FCE 在高达 50°C 的温度下保持接近最佳的加帽活性。在低于 37°C 的温度下,加帽活性会降低,但在相同的摩尔浓度下,FCE 的活性高于 VCE。此资源的链接相关产品:Faustovirus 加帽酶首页FAQsFaustovirus Capping Enzyme 对二级结构敏感吗?FAQ:Faustovirus Capping Enzyme 对二级结构敏感吗?是的。限制 5\' 端可访问性的二级结构将降低加盖产量。在较高温度下孵育封端反应或在添加封端酶之前预热样品可以帮助改善结构化底物的封端。在严重的情况下,可能需要改变转录本的序列以减少二级结构。此资源的链接相关产品:Faustovirus Capping Enzyme主页常见问题解答 Faustovirus 加帽酶 (NEB #M2081) 能否在加帽反应中使用 GTP 类似物?常见问题解答:Faustovirus 加帽酶 (NEB #M2081) 能否在加帽反应中使用 GTP 类似物?3\'-修饰的 GTP 类似物,例如 3\'-生物素-GTP (NEB #N0760S) 和 3\'-脱硫生物素-GTP (NEB #N0761S) 仅微弱地被 Faustovirus Capping Enzyme 结合。牛痘加帽酶 (NEB #M2080) 是我们针对此用例的推荐。此资源的链接相关产品:Faustovirus 加帽酶您知悉并同意,丁香通平台展示产品并非为平台自身产品,您发起询价或试用申请后,平台将会把您已提供或按要求提供的相关信息同步提供至所咨询的具体产品商家,由商家为您提供具体产品的价格咨询或产品试用注意事项及相关试用产品或完成相应购买,因此您知悉并授权平台将您的信息进行同步共享。您有权拒绝,但您知悉将无法参加询价或试用活动。丁香通为您找到 35 条 neb内切酶产品的详情参数,实时报价,价格行情,优质批发/供应等信息。您还可以找 neb内切酶,neb内切酶buff,sma1内切酶neb 等产品信息。
2016年4月12日除了每种组份都经过严格的质量监控以外,NEBNext RNA 试剂盒还都经过构建 RNA 文库或定向 RNA 文库,再在 Illumina 测序平台测序进行验证。NEBNext 试剂盒里面,各...
丁香通为您找到36条neb内切酶信息,包括neb内切酶报价行情,优质供应商,图片,品牌等最新信息,丁香通为买家提供用户服务,诚信保障等服务,批发采购neb内切酶,上丁香通。
2017年3月15日英国《自然·通讯》杂志发表一项遗传学重要研究成果,一种基因组编辑方法能够阻止小鼠视网膜退化,进而拯救失明小鼠。其所述方法利用了CRISPR/Cas9基因治疗系统,...
2012年9月17日NEB内切酶,NEB酶,NEB现货目录,NEB现货促销 标签: neb内切酶 neb产品目录 教育 neb北京促销 北京华夏远洋rna酶促 NEB官网:http://www.neb-china.com/ 北京华夏远...
2016年4月12日NEB/M5505L/现货促销/USER™ 酶,咨询热线:4006-400-850品牌 货号 产品名称 规格NEB E7530L NEBNext® Ultra™ RNA Library Prep Kit for Illumina® 96 reactio...
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发布于 : 2025-01-15
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品牌分类
Protein Expression|Protein Purification|Protein Tools
DNA Modifying Enzymes and Cloning Technologies|Next Generation Sequencing Library Preparat
DNA修饰酶
DNA Assembly Cloning and Mutagenesis Kits
DNA修饰酶和克隆技术|NGS样品制备&;目标富集|PCR
缓冲液|下一代测序文库制备|NGS样品制备&;目标富集
Protein Expression|Protein Expression & Purification Technologies|Protein Purification
表观遗传学|限制性内切酶
DNA Modifying Enzymes and Cloning Technologies|Epigenetics
Nucleic Acid Purification|RNA Reagents
表观遗传学
DNA Modifying Enzymes and Cloning Technologies
Buffers|DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplificatio
Buffers|Markers & Ladders|RNA Reagents
Next Generation Sequencing Library Preparation|NGS Sample Prep & Target Enrichment
Nucleic Acid Purification|Protein Expression & Purification Technologies|RNA Reagents
Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno
Buffers|DNA Modifying Enzymes and Cloning Technologies
Buffers|Competent Cells
DNA Modifying Enzymes and Cloning Technologies|Next Generation Sequencing Library Preparat
Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno
Buffers
Buffers|Nucleic Acid Purification
Epigenetics|Protein Expression & Purification Technologies|Protein Purification
Epigenetics|Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplific
DNA质粒和底物
Markers & Ladders|RNA Reagents
感受态细胞|蛋白质表达|蛋白质表达&;净化技术|Prot
Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno
DNA组装、克隆和突变试剂盒
DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplification Techno
Competent Cells|Protein Expression|Protein Expression & Purification Technologies|Prot
Competent Cells|Protein Expression & Purification Technologies
蛋白质工具
Epigenetics|Next Generation Sequencing Library Preparation|NGS Sample Prep & Target En
Next Generation Sequencing Library Preparation|NGS Sample Prep & Target Enrichment|PCR
Glycobiology|Protein Tools
RNA试剂
Next Generation Sequencing Library Preparation|NGS Sample Prep & Target Enrichment|PCR
Protein Expression|Protein Expression & Purification Technologies|Protein Purification
基因表达
DNA Modifying Enzymes and Cloning Technologies|RNA Reagents
Cellular Analysis|DNA Plasmids & Substrates|Protein Tools
下一代测序的样品制备
Markers & Ladders|Protein Tools
PCR,qPCR和扩增技术
DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplification Techno
限制性内切酶
主管细胞
Epigenetics|Next Generation Sequencing Library Preparation|NGS Sample Prep & Target En
DNA Modifying Enzymes and Cloning Technologies|Nucleic Acid Purification
PCR, Polymerases & Amplification Technologies
Protein Expression|Protein Expression & Purification Technologies
DNA Modifying Enzymes and Cloning Technologies|Next Generation Sequencing Library Preparat
Competent Cells|Protein Expression|Protein Expression & Purification Technologies|Prot
细胞分析
下一代测序文库制备|NGS样品制备&;目标富集|PCR
DNA质粒&;底物|蛋白质表达|蛋白质表达&;净化技术
核酸提纯
Epigenetics|NGS Sample Prep & Target Enrichment|PCR, Polymerases & Amplification T
DNA Modifying Enzymes and Cloning Technologies|Next Generation Sequencing Library Preparat
Buffers|Glycobiology
Nucleic Acid Purification|Protein Tools
NGS Sample Prep & Target Enrichment|PCR, Polymerases & Amplification Technologies|
Buffers|DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplificatio
Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno
Buffers|Competent Cells|Protein Expression|Protein Expression & Purification Technolog
Next Generation Sequencing Library Preparation|NGS Sample Prep & Target Enrichment|PCR
DNA Assembly Cloning and Mutagenesis Kits|DNA Modifying Enzymes and Cloning Technologies
Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno
Buffers|PCR, Polymerases & Amplification Technologies
DNA Modifying Enzymes and Cloning Technologies|Genome Editing
Buffers|Next Generation Sequencing Library Preparation
Next Generation Sequencing Library Preparation
Cellular Analysis|Protein Tools
PCR, Polymerases & Amplification Technologies|Protein Expression|Protein Expression &a
Genome Editing|RNA Reagents
Cellular Analysis|Epigenetics|Protein Tools
DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplification Techno
Protein Expression & Purification Technologies|Protein Purification
Buffers|DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplificatio
Competent Cells|Protein Expression|Protein Expression & Purification Technologies
DNA Plasmids & Substrates|Protein Expression|Protein Expression & Purification Tec
Buffers|RNA Reagents
标记&;梯子
DNA Plasmids & Substrates|Protein Expression|Protein Expression & Purification Tec
Nucleic Acid Purification|PCR, Polymerases & Amplification Technologies|RNA Reagents
DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplification Techno
Next Generation Sequencing Library Preparation|RNA Reagents
PCR, Polymerases & Amplification Technologies|RNA Reagents
Competent Cells|Protein Expression|Protein Expression & Purification Technologies|Prot
Protein Expression & Purification Technologies|RNA Reagents
基因组编辑
糖生物学
细胞分析|蛋白质表达&;纯化技术|蛋白质工具
Epigenetics|Next Generation Sequencing Library Preparation
DNA Plasmids & Substrates|Protein Expression|Protein Expression & Purification Tec
Buffers|Markers & Ladders|Protein Tools
DNA修饰酶和克隆技术|PCR,聚合酶&;放大技术
标记和梯子
Markers & Ladders|Nucleic Acid Purification|RNA Reagents
Buffers|Restriction Endonucleases
蛋白质表达与纯化技术
Buffers|Markers & Ladders
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