The Monarch RNA Cleanup Kit (10 µg) rapidly and reliably purifies up to 10 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design ensures zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nts).
Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.
Monarch RNA Cleanup kits are also available for 50 µg (NEB #T2040) and 500 µg (NEB #T2050) binding capacities. Columns and buffers are also available separately for convenience.
Specifications andApplications:
SPECIFICATIONS | |
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RNA Sample Type | Cleanup and concentration of RNA from enzymatic reactions (labeling, capping, in vitrotranscription reactions, DNase I treatment) |
Binding Capacity | 10 μg |
RNA Size Range | ≥ 25 nt ( ≥ 15 nt with modified protocol) |
Typical Recovery | 70–100% |
Elution Volume | 6–20 µl |
Purity | A260/280 > 1.8 and A260/230 > 1.8 |
Protocol Time | 5 minutes of spin and incubation time |
Compatible Downstream Applications | RT-PCR, Small RNAlibrary prep for NGS, RNA Library Prep for NGS |
APPLICATIONS | |
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RNA Cleanup and Concentration (including from the TRIzol aqueous phase) | RNA purified by other methods can be further purified |
Enzymatic Reaction Cleanup | Enzymes such as RNA polymerases, DNase I, Proteinase K and phosphatases are removed allowing efficient desalting |
In vitro Transcription Cleanup | Enzymes and excess NTPs are removed to yield highly pure synthesized RNA |
RNA Gel Extraction | Purification of RNA from agarose gels |
RNA Fractionation | Fractionation of RNA into small and large RNA pools |
- This product is related to the following categories:
- RNA Cleanup Products,
- RNA Reagents Products,
- RNA Extraction & Purification Products, Nucleic Acid Purification Products
- This product can be used in the following applications:
- RNA Purification and Isolation,
- RNA Analysis